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Post Info TOPIC: need help understanding test results
mallani


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RE: need help understanding test results
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Hi Wanda,

This has been the subject of previous discussion. Drug companies are very secretive about the 'targets' used in VL and Genotyping PCR tests. The older tests, using just one target from the 5'UTR of the virus, often resulted in patients being told they had 'Genotype 1a/b' . Some patients were retested, and found they had changed from 1b to 1a.

The only reliable test is a line probe assay, using targets from 2 sites in the 5'UTR and from NS5B. INNO-LiPA and Abbott have these, and the results won't be confusing.

Get your doctor to check, and retest with a Lab. that has these tests available. For a VL test, only one target is required. Cheers.



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Geno 1b, IL28B CT,  x3 prior relapser,  ex-cirrhotic, 75 yo, did 48 weeks with Victrelis/Peg./Riba.  VL 1.28m at start, UNDET. at 8 ,12 ,16 ,24 ,30  and 48 weeks.  EOT 15 Feb 2013 , UNDET. at EOT + 28 weeks. SVR!  Still Undet. at EOT +5 years

Malcolm
wanthib


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Tig56 wrote:

I agree with Duane on this one. After reviewing those two reports, it seems obvious that the testing procedure for the second genotyping process was/is flawed. I'd either go with the first determination or have the genotyping repeated utilizing an alternative method. After reading those reports, I have to question the intelligence of them even using that form of genotyping. This is the first I've heard of this and until this report, I had never seen such confusion. Nice find Duane!

Tig

Thank you I will go with the first test results. I'm not sure why she wanted to repeat the test anyway because we gave her copies of all previous test results. 



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Tig


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I agree with Duane on this one. After reviewing those two reports, it seems obvious that the testing procedure for the second genotyping process was/is flawed. I'd either go with the first determination or have the genotyping repeated utilizing an alternative method. After reading those reports, I have to question the intelligence of them even using that form of genotyping. This is the first I've heard of this and until this report, I had never seen such confusion. Nice find Duane!

Tig



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Tig

68yo GT1A - 5 Mil - A2/F3 - (1996) Intron A - Non Responder, (2013) Peg/Riba/Vic SOT:05/23/13 EOT:12/04/13 SVR 9+ years!

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wanthib


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Duane,

Thanks for your reply and the links. So the problem is with the method that they used to identify the subtype.



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Dzdayscomin


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wanthib wrote:

Hi My husband was diagnosed with hcv in 1999 has never treated. He had a genotype test in 2008 that show he is 1b.  He is seeing a new hepatologist and she order labs in order to start treatment on the new tx coming out Oct 10th. We picked up copy of the labs today the genotype results have me confused , what does this mean?  Hvc Genotype by 1a or 1b cannot be further subtyped into 1a or 1b due to high conservation of the 5' untranslated region of the hcv genome. In addition, type 6 virus may be misclassified as type 1 in some cases.

 http://jcm.asm.org/content/40/9/3127.full

 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785465/

I would go with the 1st test i don't  know why they went the direction they did in sub typing method?

Some links to help

Duane



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wanthib


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Hi My husband was diagnosed with hcv in 1999 has never treated. He had a genotype test in 2008 that show he is 1b.  He is seeing a new hepatologist and she order labs in order to start treatment on the new tx coming out Oct 10th. We picked up copy of the labs today the genotype results have me confused , what does this mean?  Hvc Genotype by 1a or 1b cannot be further subtyped into 1a or 1b due to high conservation of the 5' untranslated region of the hcv genome. In addition, type 6 virus may be misclassified as type 1 in some cases.



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